How to cite item

Antisense IRAK-2 oligodeoxynucleotide inhibits interleukin-1-induced nuclear factor-kappa B activation in vitro

   
@article{APS7996,
	author = {Fu-Kun Guo and Yi-Lei Li and Shu-Guang Wu},
	title = {Antisense IRAK-2 oligodeoxynucleotide inhibits interleukin-1-induced nuclear factor-kappa B activation in vitro},
	journal = {Acta Pharmacologica Sinica},
	volume = {21},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {\"AIM:
To study the inhibitory effects of antisense interleukin-1 receptor associated kinase-2 (IRAK-2) oligodeoxynucleotide (ODN) on interleukin-1 (IL-1)-stimulated nuclear factor-kappa B (NF-kappa B) activation.
METHODS:
Antisense IRAK-2 ODN was delivered by lipofectin encapsulation into human embryonic kidney 293 cells (HEK 293 cells). The levels of NF-kappa B were assayed by sandwich ELISA.
RESULTS:
(1) Treatment of HEK 293 cells with IL-1 enhanced NF-kappa B level in nuclei by 518.5% +/- 2.1%. (2) Antisense IRAK-2 ODN inhibited IL-1-induced NF-kappa B activation in a concentration (1-8 micrograms)- and time (5-24 h)-dependent manner. A maximum inhibition was 70.7% +/- 1.0% from Acontrol 0.834 +/- 0.014 to 0.244 +/- 0.008 after treatment with antisense IRAK-2 ODN 4 micrograms for 8 h.
CONCLUSION:
Antisense IRAK-2 ODN inhibited IL-1-induced NF-kappa B activation.\"},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7996}
}