@article{APS7993,
author = {Pei Hu and Ji Jiang and Hong-Hong Xie},
title = {Determination of idebenone in plasma by HPLC/MS},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {4},
year = {2016},
keywords = {},
abstract = {\"AIM:
To develop a sensitive method for the analysis of idebenone [Ide, 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-p-benzoquinone] in human plasma.
METHODS:
The concentrations in plasma were determined by a high performance liquid chromatography-mass spectrometry coupled with an atmosphere pressure chemical impact ion source method (LC/MS-APCI).
RESULTS:
The chromatograms indicated a good separation of the analytes and there was no interference by other compounds. The coefficients of determination of the calibration curves were above 0.999. The calibration range was 20-600 micrograms.L-1. Peak plasma idebenone concentration (Cmax) of (316 +/- 85) micrograms.L-1 was achieved within (96 +/- 34) min (Tpeak) after an oral dose of 30 mg. Lower limit of quantitation of the method for Ide determination was 20 micrograms.L-1.
CONCLUSION:
The advantages of using LC/MS-APCI technique include better sensitivity, higher selectivity, and less time consumption, compared with HPLC method.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7993}
}