@article{APS7991,
author = {Wei Ou-Yang and Ya-Lin Huang and Cui-Di Da and Jie-Shi Cheng},
title = {Alteration of basic fibroblast growth factor expression in rat during cerebral ischemia},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {4},
year = {2016},
keywords = {},
abstract = {\"AIM:
To investigate the distribution and expression changes of basic fibroblast growth factor (bFGF)-like immunoreactivity (IR) in focal cerebral ischemic rat brain.
METHODS:
The expression of bFGF was observed by fluoro-immunohistochemistry. The focal cerebral ischemic injury was carried out using middle cerebral artery occlusion (MCAO) model.
RESULTS:
Confocal images demonstrated that both the striatum and the frontoparietal cortex showed increases of bFGF-like IR after 2-h MCAO and 24-h reperfusion. In striatum, the increase patterns of bFGF-like IR were different according to the ischemic extent. In the core of infarct and its surrounding region (with more IR induction), bFGF-like IR was mainly located in astrocytes. In region adjacent to infarct (with most IR induction, the grey ratio of bFGF-like IR increased from sham-98% +/- 10% to ischemia-125% +/- 6%), some neurons also showed an upregulation of bFGF-like IR. In frontoparietal cortex, strong induction of bFGF-like IR was mostly seen in neurons (The grey ratio of bFGF-like IR increased from sham-104% +/- 11% to ischemia-132% +/- 28%), although it was observed in astrocytes as well.
CONCLUSION:
The expression of bFGF increased after focal cerebral ischemia in rats, suggesting that: in striatum, astrocytes may play an important role in the protection of neurons via the overexpression of bFGF; whereas in cortex, neurons probably exert an autoprotection through expressing bFGF themselves.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7991}
}