@article{APS7902,
author = {Jie Li and Liang-Zhong Xu and Jin-Juan Yao and Wei-Jian Guo and Peng Xia and Ying Chen},
title = {Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: To study the reversal effects of droloxifene (DRO) on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR).
Methods: K562 cell line resistant to ADR (K562/A02) and K562 cell line sensitive to ADR (K562) were treated with DRO. Using MTT assay, chemosensitivity to ADR in DRO-treated K562 cell lines was studied. Before and after the treatment with DRO 10 micromol/L, MDR1 and GSTpi gene expression were assayed by reverse transcription-polymerase chain reaction and immunocytochemistry assay. Flow cytometry was used to determine intracellular ADR concentration.
Results: DRO significantly reversed MDR in K562/A02 (P < 0.01). After treatment of DRO 20, 10, and 5 micromol/L, the chemosensitivity to ADR was increased to 14, 13, and 4 folds, respectively. The reversal activity of DRO was similar to that of verapamil (VRP). After treated with DRO 10 micromol/L, both MDR1 and GSTpi mRNA expression began to decline on the 2nd day, and significantly decreased on the 5th day (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7902}
}