@article{APS7896,
author = {Jia-hua HU and Na YANG and Ying-hua MA and Jie JIANG and Jin-fu ZHANG and Jian FEI and Li-he GUO},
title = {Identification of glutamate transporters and receptors in mouse testis},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the presence of glutamate transporters and receptors in mouse testis.
METHODS:
Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis.
RESULTS:
Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 +/- 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0+/-1.6) micromol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis.
CONCLUSION:
Glutamate transporters and receptors do exist in mouse testis.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7896}
}