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Doxepin protects cultured neurons against oxidative stress-induced injury

   
@article{APS7885,
	author = {Bian-sheng JI and He JI and Guo-qing LIU},
	title = {Doxepin protects cultured neurons against oxidative stress-induced injury},
	journal = {Acta Pharmacologica Sinica},
	volume = {25},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {AIM:
To investigate protective effects of doxepin (Dox) on cultured neuronal injury induced by oxidative stress.
METHODS:
Cytotoxicities of glutamate (Glu 0.5 mmol/L, 15 min), sodium dithionite (0.5 mmol/L, 24 h) or hemoglobin (Hb100 mg/L,24 h)and the protective effects of Dox were observed.
RESULTS:
Exposure of cultured neurons to Glu, sodium dithionite and Hb developed a neurotoxicity expressed in the thiazol blue tetrazolium bromide (MTT) assay, the increase of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and intracellular [Ca2+]i accumulation, as well as the decrease of superoxide dismutase (SOD) activity. DOX 1-100 nmol/L significantly inhibited all above changes.
CONCLUSION:
Dox protects cultured neurons against oxidative stress-induced injury by suppressing intracellular [Ca2+]i accumulation, decreasing lipid peroxide generation and stimulating antioxidant enzyme.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7885}
}