@article{APS7860,
author = {Jun-lin ZHOU and Xiao-guang ZHU and Yi-ling LING and Qing LI},
title = {Melatonin reduces peroxynitrite-induced injury in aortic smooth muscle cells},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM:
To study the protective role of melatonin (MT) in peroxynitrite-induced injury in cultured aortic smooth muscular cells (ASMC).
METHODS:
Peroxynitrite was synthesized chemically with a quenched flow reaction. Cells were exposed to peroxynitrite 500 micromol/L for 1 h in the absence or presence of various concentrations of MT 100, 300, and 500 micromol/L. Nitrotyrosine (NT), a specific \"footprint\"of peroxynitrite formation, was detected by immunohistochemical technique. The DNA damage was assayed by TUNEL technique. The levels of MDA in the medium and cell viability were measured.
RESULTS:
Incubation of ASMC with peroxynitrite 500 micromol/L for 1 h elicited the increase in the extent of immunostaining for NT, the rate of the TUNEL-positive cell, the content of MDA in the medium, and the number of dead cell. Pretreatment of ASMC with MT 100-500 micromol/L decreased these peroxynitrite-induced changes in a concentration-dependent manner.
CONCLUSION:
MT attenuated the injury induced by peroxynitrite in ASMC.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7860}
}