@article{APS7841,
author = {Qing-sheng XUE and Bu-wei YU and Ze-jian WANG and Hong-zhuan CHEN},
title = {Effects of ketamine, midazolam, thiopental, and propofol on brain ischemia injury in rat cerebral cortical slices},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM:
To compare the effects of ketamine, midazolam, thiopental, and propofol on brain ischemia by the model of oxygen-glucose deprivation (OGD) in rat cerebral cortical slices.
METHODS:
Cerebral cortical slices were incubated in 2 % 2,3,5-triphenyltetrazolium chloride (TTC) solution after OGD, the damages and effects of ketamine, midazolam, thiopental, and propofol were quantitatively evaluated by ELISA reader of absorbance (A) at 490 nm, which indicated the red formazan extracted from slices, lactic dehydrogenase (LDH) releases in the incubated supernate were also measured.
RESULTS:
Progressive prolongation of OGD resulted in decreases of TTC staining. The percentage of tissue injury had a positive correlation with LDH releases, r=0.9609, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7841}
}