@article{APS7733,
author = {Ping LIU and Cheng-Hai LIU and Hai-Nan WANG and Yi-Yang HU and Cheng LIU},
title = {Effect of salvianolic acid B on collagen production and mitogen-activated protein kinase activity in rat hepatic stellate cells.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the mechanism of salvianolic acid-B (SA-B) action against
liver fibrosis relating to mediating hepatic stellate cell (HSC) activation and
transforming growth factor-beta1(TGF-beta1) intracellular signal transduction.
METHODS: HSC was isolated from normal rat through in situ perfusion of liver with
pronase E and density-gradient centrifugation with 11 % nycondenz, then cells
were subcultured. Cell proliferation was observed by [3H]TdR uptake. Cellular
collagen deposition was measured with Ponceau S stain and semi-quantified with
image analytic system. Type I collagen secretion in the supernatant was detected
with ELISA. The gene expression of type I pro-collagen was analyzed by RT-PCR.
The supernatant was acidified and active TGF-beta1 contents were assayed with
ELISA. Mitogen-activated protein kinase (MAPK) activity was analyzed with
immunoprecipitation and Western blot.
RESULTS: SA-B 0.1, 1, 10, and 100 micromol/L suppressed HSC proliferation
concentration-dependently as determined by [3H]TdR uptake by 94.1 %, 82.4 %, 62.7
%, and 4 % of the control respectively (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7733}
}