@article{APS7680,
author = {Gui-ying Cui and Jin-ming Li and Hong Cui and Li-ying Hao and Dong-ju Liu and Ke-yi Zhang},
title = {Effects of calcium channel blockers on calcium release-activated calcium currents in rat hepatocytes},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM:
To study the influences of calcium channel blockers on calcium release-activated calcium currents (ICRAC) in rat hepatocytes.
METHODS:
Whole-cell patch-clamp technique was used.
RESULTS:
The peak amplitude of ICRAC was -0.41 nA +/- 0.09 nA (n = 15), its reversal potential was about 0 mV. Verapamil (Ver), diltiazem (Dil), and nifedipine (Nif) decreased ICRAC strikingly, without affecting its reversal potential. The inhibitory rate of Ver 5 mumol.L-1 was 40% +/- 12% (n = 3), Ver 50 mumol.L-1 reduced the peak amplitude of ICRAC from -0.49 nA +/- 0.12 nA to -0.20 nA +/- 0.09 nA (P < 0.01 vs control, n = 5). The inhibitory rate was 57% +/- 15%. Dil 50 mumol.L-1 and Nif reduced ICRAC from -0.43 nA +/- 0.10 nA to -0.29 nA +/- 0.07 nA (P < 0.01 vs control, n = 5), from -0.32 nA +/- 0.08 nA to -0.27 nA +/- 0.08 nA (P < 0.01 vs control, n = 5). The inhibitory rate was 31% +/- 11%, 19% +/- 7%, respectively. The amplitude of ICRAC was dependent on extracellular Ca2+ concentration. The peak amplitude of ICRAC was -0.21 nA +/- 0.08 nA (n = 3) in Tyrode's solution with Ca2+ 1.8 mmol.L-1 (P < 0.01 vs the peak amplitude of ICRAC in external solution with Ca2+ 10 mmol.L-1).
CONCLUSION:
The three calcium antagonists inhibited ICRAC effectively and protected hepatocytes from calcium overload via the inhibition of ICRAC.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7680}
}