@article{APS7667,
author = {Chun-Yan TIAN and Chang-Qi HU and Guang XU and Hou-Yan SONG},
title = {Assessment of estrogenic activity of natural compounds using improved E-screen assay.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To improve E-screen assay and make it more accurate to screen estrogenic
compounds.
METHODS: Estrogen receptor antisense RNA expression plasmid (pCASER) was
constructed and introduced into MCF-7 with lipofectAMINE(TM), and positive clones
were screened out with G418. PCR amplification was employed to identify whether
estrogen receptor (ER) cDNA fragment had been inserted into MCF-7 cell genomes.
Western blot was applied to detect the expression of ER. Cell growth was
determined by MTT assay.
RESULTS: One ER antisense clone (MTASER) had been screened out. The effects of
17beta-estradiol, genistein, droloxifen, miyabenol C, and kobophenol A on MCF-7
were stronger than those effects on MTASER. Epidermal growth factor (EGF) had
equivalent stimulatory effects on the proliferation of MCF-7 and MTASER.
CONCLUSION: The improved E-screen assay could screen estrogenic compounds more
accurately than original E-screen assay did.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7667}
}