@article{APS7658,
author = {Zhi-Yong Liao and Yue-Hua Tang and Ming-Hua Xu and You-Min Feng and Shang-Quan Zhu},
title = {B9-serine residue is crucial for insulin actions in glucose metabolism},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: To explore the importance of B9 and B10 amino acid residues in the insulin molecule.
Methods: The [B9Glu, B10Asp] insulin (E,D-insulin) receptor binding activity, glucose upta ke activity, and lipogenesis activity were measured in isolated adipocytes. The translocation of glucose transporter 4 (Glut4) and the phosphorylation of insulin receptor (IR) stimulated by E, D-insulin were determined by Western blotting.
Results: Compared with native insulin, the receptor binding activity of E,D-insulin was 31 %; the stimulating activity of E,D-insulin in glucose transport and lipogenesis were 45 % and 40 % respectively; the stimulations of Glut4 translocation and insulin receptor autophosphorylation of E,D-insulin were about 58 % and 46 % respectively.
Conclusion: B9-serine residue is crucial for insulin actions in glucose metabolism.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7658}
}