@article{APS7622,
author = {Bai-Yan LI and John H SCHILD},
title = {Comparisons of somatic action potentials from dispersed and intact rat nodose ganglia using patch-clamp technique.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To differentiate the electrophysiological characteristics of somatic action
potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those
from slices of intact Juv and adult rat nodose ganglia.
METHODS: For isolated cell recordings nodose ganglia from 3-8 d old Neo and 4
weeks old Juv rats were dissociated using trypsin and collagenase, respectively.
Nodose ganglia slices with attached vagus were prepared using a sequential
treatment of collagenase and trypsin for both Juv and adult rats. Conduction
velocity (CV) was collected by vagal stimulation. Whole-cell patch was applied
for somatic AP recordings.
RESULTS: (1) 281 NSN from both isolated cells and nodose slices were studied.
Across all age groups, there was no difference observed among either C- or
A-types. The difference between C- and A-type was significant. (2) Neurons
exhibiting AP with prominent repolarization hump, broader APD50 (>2.0 ms),
upstroke velocity at the point of APD50 (UV(APD50)) and downstroke velocity at
the point of APD50 (DV(APD50)) below 100 V . s-1 and 50 V . s-1 were classified
as C-type (n = 222). Those without a hump, brief APD50 (},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7622}
}