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Effect of dl-praeruptorin A on ATP sensitive potassium channels in human cortical neurons

  
@article{APS7598,
	author = {Shen-Li Zhang and Jin-Ming Li and Qing-Heng Xiao and An-Hua Wu and Qing Zhao and Guo-Rui Yang and Ke-Yi Zhang},
	title = {Effect of dl-praeruptorin A on ATP sensitive potassium channels in human cortical neurons},
	journal = {Acta Pharmacologica Sinica},
	volume = {22},
	number = {9},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the effect of dl-praeruptorin A (Pd-Ia) on ATP sensitive potassium channels (KATP channel) in human cortical neurons.
Methods: Using standard whole cell recording method. Cell membranes were held at -40 mV, commanding potential was -30 to +100 mV and duration was 600 ms.
Results: Pd-Ia activated KATP channels in human cortical neurons in a concentration-dependent manner. After consecutive perfusion with external solution containing Pd-Ia 0.001, 0.01, 0.1, and 1 micromol/L, currents increased from control (0.9 +/- 0.4) nA to (1.0 +/- 0.4) nA, (1.1 +/- 0.4) nA, (1.2 +/- 0.4) nA, and (1.3 +/- 0.4) nA (P < 0.05 or P < 0.01, n = 5) respectively. Then the current decreased to (0.90 +/- 0.37) nA (P < 0.01, n = 5) after washout with glibenclamide (10 micromol/L). The increscent part of the currents could nearly be inhibited by specific KATP channel inhibitor.
Conclusion: Pd-Ia could open KATP channel and it is a kind of potassium channel opener},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7598}
}