@article{APS7588,
author = {Lu-Ping Kang and Li-Hong Qi and Jun-Ping Zhang and Ning Shi and Min Zhang and Tang-Ming Wu and Jing Chen},
title = {Effect of genistein and quercetin on proliferation, collagen synthesis, and type I procollagen mRNA levels of rat hepatic stellate cells},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {9},
year = {2016},
keywords = {},
abstract = {Aim: To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells.
Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by [3H]proline incorporation assay. Type I mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR).
Results: GE (25 - 70 micromol/L) and QU (6.25 - 50 micromol/L) concentration-dependently reduced serum-driven HSC-T6 cell proliferation and collagen synthesis associated with a suppression of type I procollagen mRNA level.
Conclusion: GE and QU inhibited hepatic stellate cell proliferation and collagen synthesis that might have a protective role against liver fibrosis.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7588}
}