@article{APS7446,
author = {Qing Huang and Lan Shao and Hong Jiang and Zhen-Chuan Miao and Qing-De Shi and Shu-Sen Liu},
title = {Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To observe the effects of insulin on liver mitochondrial respiratory function, activity of H+-ATPase, and superoxide anion free radicals production in diabetic rats.
Methods: Rats were injected iv with alloxan 40 mg/kg to induce diabetes. The liver mitochondrial respiratory function was assayed by measurement of oxygen consumption using a Clark oxygen electrode. Superoxide anion production was assayed using chemiluminescence method. Activities of H+-ATPase were measured by luciferin-luciferase system and inorganic phosphorus's method.
Results: Insulin 1 U/kg sc daily for 9 weeks improved oxidative phosphorylation, respiratory rate state 3 (P < 0.05), respiratory control ration (P < 0.01), and ADP:O ratio (P < 0.01), but there were no obvious effect on respiratory rate state 4 (P > 0.05). In the insulin group, synthesis activity of H+-ATPase was obviously increased (P < 0.05) and hydrolytic activity of H+-ATPase was remarkably decreased (P < 0.01), compared with the diabetes group. Insulin 1 U/kg for 9 weeks apparently decreased the production of O2.- (P < 0.01) in liver mitochondria of diabetic rats.
Conclusion: Insulin can prevent the injury from superoxide anion in liver mitochondria, and improve the function of the liver mitochondria oxidative phosphorylation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7446}
}