@article{APS7435,
author = {Hui-jun Yuan and Wei-yuan Yu and Cheng-hua Shi and Man-ji Sun},
title = {Characteristics of recombinant human butyrylcholinesterase},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM:
To study the biochemical-pharmacological properties of the recombinant human butyrylcholinesterase (rhBChE) and thereby to size up the potential possibility of using it as a detoxifying agent in succinylcholine intoxication.
METHODS:
CHO-dhfr cells were transfected with plasmids by electroporation. BChE activity was determined colorimetrically by 5, 5'-dithiobis-(2-nitrobenzoic acid) (DTNB) method. Antigenicity was estimated by enzyme-linked immunosorbent assay and Western blot.
RESULTS:
The maximal expression amounted to 25.83 ng.h-1/10(6) cells. The rhBChE was highly similar to the native human BChE (nhBChE) in terms of its catalytic property, substrate affinity, inhibitor sensitivity, reactivation, stability, and immunoreactivity with anti-nhBChE antibodies. Mice challenged with 1.5 lethal dose of succinylcholine preincubated with rhBChE survived without any symptoms of intoxication.
CONCLUSION:
The rhBChE and nhBChE exhibit similar biochemical-pharmacological features. It is of potential value in practical use.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7435}
}