@article{APS7423,
author = {Xin-tang Zhang and Shi-yin Yao and Ying-gao Xu and Shang-quan Zhu},
title = {Binding characteristics of pancreatic polypeptide receptors on rat hepatic membranes},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM:
To study the binding characteristics of pancreatic polypeptide (PP) receptors on rat hepatic membranes.
METHODS:
125I-PP suitable to study interaction between ligand and receptors were prepared. 125I-porcine PP and 125I-duck PP were used to study PP receptor binding in the controlled conditions.
RESULTS:
The binding of 125I-porcine PP to receptors on rat hepatic membranes was time- and temperature-dependent. The specific binding of 125I-porcine PP was inhibited by unlabeled porcine PP in a concentration-dependent manner, whereas duck PP was only partially inhibited in the high concentration (> 500 nmol.L-1). Scatchard analysis produced a curvilinear plot, suggesting multiple affinity binding sites, i.e., high-affinity and low-affinity with dissociation constants (Kd) 5.4 and 158 nmol.L-1, respectively.
CONCLUSION:
Rat hepatic membranes possessed specific PP receptors and porcine PP binding activity was much higher than that of duck PP.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7423}
}