@article{APS7400,
author = {Li Zhang and Yao-Cheng Rui and Yan Qiu and Tie-Jun Li},
title = {Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304).
Methods: VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization.
Results: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time- and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells.
Conclusion: LPC could induce a strong expression of VEGF in ECV304 cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7400}
}