@article{APS7338,
author = {Zhi-Hong Liu and Ying-Jian Li and Chao-Hong Chen and Dong Liu and Lei-Shi Li},
title = {Glucose transporter in human glomerular mesangial cells modulated by transforming growth factor-beta and rhein},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: To identify GLUT1 in human mesangial cells and its regulation by TGF-beta1 and rhein.
Methods: Identification of GLUT1 was performed in human mesangial cells by analyzing its mRNA expression, protein product, and functional assay. The effects of TGF-beta1 and rhein on glucose uptake and GLUT1 mRNA expression in mesangial cells were examined by [3H]-2-deoxy-D-glucose uptake and Northern blotting analysis.
Results: It was found that human mesangial cells do express functional GLUT1. The 2-DOG uptake of mesangial cells was markedly increased by TGF-beta1 stimulation with simultaneous elevated expression of GLUT1 mRNA. Increased glucose uptake and GLUT1 mRNA expression in mesangial cells induced by TGF-beta1 were markedly attenuated by rhein in a dose-dependent manner.
Conclusion: We found that functional GLUT1 did present in human mesangial cells. TGF-beta1 stimulated the glucose uptake in mesangial cells through upregulation of GLUT1 expression, this effect of TGF-beta1 could be antagonized by rhein.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7338}
}