@article{APS7322,
author = {Bo Ding and Shao-Ling Huang and Shi-Qin Zhang and Yun-Xia Li},
title = {Effect of PKC-zeta mediating Ang II-stimulated activation of CCDPK on rat cardiac fibroblast proliferation},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {2},
year = {2016},
keywords = {},
abstract = {\"AIM:
To investigate whether the effect of angiotensin (Ang) II or epidermal growth factor (EGF) on cardiac fibroblast proliferation involved in activation of extracellular signal-regulated kinase (ERK) 1/2 or Ca(2+)-calmodulin dependent protein kinase(CCDPK) mediated by protein kinase C (PKC)-zeta.
METHODS:
Relative activity of CCDPK was measured by Western blotting. DNA synthesis was assayed by [3H]thymidine incorporation.
RESULTS:
PDBU caused no decrease in Ang II- and 10% FCS-stimulated CCDPK activity and DNA synthesis. In contrary, 65% or 75% EGF- or tetradecanoylphorbol acetate (TDPA, formally called PMA)--stimulated CCDPK activity and 38% or 42% [3H]thymidine incorporation treated by PDBU were inhibited, respectively. Meanwhile 70% and 72% CCDPK activities induced by Ang II and EGF were inhibited by PD 98059, respectively.
CONCLUSION:
PKC-zeta mediated Ang II-induced activation of CCDPK and cardiac fibroblast proliferation.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7322}
}