@article{APS7310,
author = {Shi-Xi Zheng and Li-Jun Zhou and Zhong-Liang Chen and Meng-Long Yin and Xing-Zu Zhu},
title = {Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {2},
year = {2016},
keywords = {},
abstract = {\"AIM:
To study the effects of bilobalide on the expression of glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro.
METHODS:
Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expression in the astrocytes after bilobalide (5, 15, 50, 100 mumol.L-1) treatment. Immunohistochemistry method was used to detect GDNF and VEGF protein expression in cells treated with bilobalide 50 mumol.L-1 for 24 h.
RESULTS:
GDNF and VEGF mRNA increased markedly after astrocytes were treated with bilobalide 50 mumol.L-1 for 12 h. GDNF and VEGF protein were detected in the cytoplasm of astrocytes after the cells were treated with bilobalide 50 mumol.L-1 for 24 h.
CONCLUSION:
Bilobalide induced GDNF and VEGF expression in the cultured astrocytes.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7310}
}