@article{APS7272,
author = {Zhi-Fei WANG and Jin ZHOU and Xi-Can TANG},
title = {Huperzine B protects rat pheochromocytoma cells against oxygen-glucose deprivation-induced injury.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {12},
year = {2016},
keywords = {},
abstract = {AIM: To test the ability of huperzine B (HupB) to alleviate injury from
oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.
METHODS: After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was
observed by phase-contrast microscopy; cell survival was quantified by the
reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric
acid; superoxide dismutase (SOD) was assayed by a modification of the
xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and
Weil.
RESULTS: OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of
cells, along with major changes in morphology and biochemistry including elevated
level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100
micromol/L for 2 h showed significantly improved survival and reduced biochemical
and morphologic signs of toxicity.
CONCLUSION: HupB protected PC12 cells against OGD-induced injury, most likely by
alleviating disturbances of oxidative and energy metabolism.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7272}
}