@article{APS7243,
author = {Li-Jun Zhou and Wei Song and Xing-Zu Zhu and Zhong-Liang Chen and Meng-Long Yin and Xiao-Fang Cheng},
title = {Protective effects of bilobalide on amyloid beta-peptide 25-35-induced PC12 cell cytotoxicity},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To study the effect of bilobalide, a terpene extracted from the leaves of Ginkgo biloba, on beta-amyloid peptide fragment 25-35 (A beta 25-35)-induced PC12 cell cytotoxicity.
METHODS: 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assay were used to measure the viability of PC12 cells. Thiobarbituric acid-reactive substances were measured to determine lipid peroxidation of cells. Antioxidant enzymes in PC12 cells were detected.
RESULTS: Treatment of PC12 cells with A beta 25-35 (100 mumol.L-1) for 24 h caused a great decrease in cell viability (P < 0.01 compared with control). Bilobalide 25-100 mumol.L-1 dose-dependently attenuated the cytotoxic effect of A beta 25-35. Bilobalide also inhibited A beta 25-35 (100 mumol.L-1)-induced elevation of lipid peroxidation and decline of antioxidant enzyme activities.
CONCLUSION: Bilobalide protected PC12 cells from A beta 25-35-induced cytotoxicity.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7243}
}