@article{APS7240,
author = {Rong ZHOU and De-Yao HU and Liang-Ming LIU and Xue-Wu ZHOU},
title = {Protective effects of apocynin on \"two-hit\" injury induced by hemorrhagic shock and lipopolysaccharide.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {11},
year = {2016},
keywords = {},
abstract = {AIM: To evaluate the protective effects of apocynin on \"two-hit\" injury in rats.
METHODS: \"Two-hit\" injury model of rat was induced by hemorrhagic shock (40 mmHg
for 45 min) followed by iv administration of lipopolysaccharide (LPS, 150
microg/kg). Rats were randomized into seven groups: Sham, LPS, hemorrhage,
hemorrhage/LPS, and hemorrhage/LPS+apocynin (2.5, 5.0, and 10.0 mg/kg). Apocynin
was dissolved in the resuscitation fluid (normal saline, NS) and administered iv
for 2 h. After LPS or NS administration, the survival rates at 8 h, 16 h, 24 h,
and 48 h were monitored. The content of malondialdehyde (MDA) was measured in
lung at 3 h and 6 h after iv LPS and in serum before hemorrhage, after
hemorrhage, and at 0, 0.5, 1, 2, 4, and 6 h after iv LPS. Myeloperoxidase (MPO)
activity in lung and liver was examined at 3 h and 6 h after iv LPS/NS.
RESULTS: After \"two-hit\" injury, the survival rates of rats at 8 h, 16 h, 24 h,
and 48 h were 64.3 %, 35.7 %, 28.6 %, and 14.3 % respectively, there were
significant differences as compared to sham group (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7240}
}