@article{APS7221,
author = {Xuan Liu and Shi-Xi Zheng and Li-Jun Zhou and Xing-Zu Zhu},
title = {Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To examine the effect of recombinant human basic fibroblast growth factor (bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes.
METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively.
RESULTS: Treatment with bFGF dose-dependently increased the VEGF mRNA level in astrocytes. The up-regulation of VEGF mRNA induced by bFGF (10 micrograms/L) was detected as short as 3-h treatment. The increase of VEGF mRNA level reached the maximum after 24-h treatment with bFGF. The immunocytochemical staining showed that the VEGF protein level in astrocytes also increased after the cells were incubated with bFGF.
CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulating VEGF expression in astrocytes.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7221}
}