@article{APS7131,
author = {Thomas V BILFINGER and James A VOSSWINKEL and Patrick CADET and Cristos M RIALAS and Harold I MAGAZINE and George B STEFANO},
title = {Direct assessment and diminished production of morphine stimulated NO by diabetic endothelium from saphenous vein.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM: To directly measure in real time basal and stimulated levels of NO released
from human saphenous vein endothelium and to quantify the expression of the mu
opiate receptor, which has been linked with NO release.
METHODS: Saphenous vein segments from patients with type 2 diabetes (n=12) and
patients without diabetes (n=8) were obtained. The release of NO was measured
directly from the endothelium using a NO-specific amperometric probe.
N(Omega)-nitro-L-arginine methyl ester (L-NAME, 0.1 mmol/L), a NO synthase (NOS)
inhibitor, or morphine (1 mumol/L), a stimulant, was administered and the
measurements were repeated. Values were reported relative to the mean initial
measurement of NO release from diabetic endothelium, which was defined as the
relative zero level of NO release. A RT-PCR was then performed on the endothelium
to measure mu opiate receptor expression.
RESULTS: Diabetic patients (n=12) showed a relative and significantly diminished
basal level of released NO, (0.049+/-0.012) nmol/L, compared with non diabetic
patients (n=8), (0.42+/-0.12) nmol/L (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7131}
}