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Inhibition of 11beta-hydroxysteroid dehydrogenase in guinea pig kidney by three bioflavonoids and their interactions with gossypol.

  
@article{APS7130,
	author = {Mao-Shan WANG and Hong SHI and Ke-Shen WANG and Marcus M REIDENBERG},
	title = {Inhibition of 11beta-hydroxysteroid dehydrogenase in guinea pig kidney by three bioflavonoids and their interactions with gossypol.},
	journal = {Acta Pharmacologica Sinica},
	volume = {23},
	number = {1},
	year = {2016},
	keywords = {},
	abstract = {AIM: To study the effects of some bioflavonoids on the gossypol-induced
hypokalemia.
METHODS: The 11beta-hydroxysteroid dehydrogenase (11beta-OHSD) protein was
prepared from guinea pig kidney. The activity of 11beta-OHSD with NAD as the
coenzyme was measured by HPLC. The drug interaction was analysed by
isobolographic method.
RESULTS: The 11beta-OHSD can be inhibited by some bioflavonoids. The IC50 (95 %
confidence limits) values were: quercetin 164 (79 - 341) micromol/L, morin 913
(385 - 2173) micromol/L, and naringenin 2193 (1114 - 4315) micromol/L. When the
11beta-OHSD was treated with quercetin, tangeretin, morin, naringenin plus
gossypol, the combination index (CI) values were 0.92, 0.85, 0.98, and 1.01
respectively.
CONCLUSION: The interaction of some bioflavonoids with gossypol might be one of
the factors for gossypol-induced hypokalemia.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7130}
}