@article{APS7112,
author = {Qi-Yang He and Hong-Qing Zhang and Da-Ben Pang and Xu-Sheng Chi and Shao-Bai Xue},
title = {Resistance to apoptosis of harringtonine-resistant HL60 cells induced by tetrandrine},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To study the mechanism of resistance to apoptosis in the harringtonine (Har)-resistant HL60 cells with tetrandrine (Tet).
METHODS: Growth inhibition, flow cytometry, DNA agarose gel electrophoresis, protein phosphorylation, and RNA dot hybridization.
RESULTS: The resistant cells had no cross resistance to Tet. Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis. The high phosphorylation of protein < 30 kDa occurred when the resistant cells were treated with Tet. Tet and Har increased the expression of c-myc mRNA in the sensitive HL60 cells. The expression of c-myc mRNA in the resistant cells was obviously decreased and almost not changed in treatment with Tet and Har.
CONCLUSION: Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis, and the resistance to apoptosis induced by Tet was associated with the high protein phosphorylation and reduction of the expression of c-myc mRNA.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7112}
}