How to cite item

Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes

   
@article{APS7039,
	author = {Lei-lei Chen and Jun-chao Wu and Lin-hui Wang and Jin Wang and Zheng-hong Qin and Marian Difiglia and Fang Lin},
	title = {Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes},
	journal = {Acta Pharmacologica Sinica},
	volume = {33},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {Aim:  To investigate the effects of rapamycin on glutamate uptake in cultured rat astrocytes expressing N-terminal 552 residues of mutant huntingtin (Htt-552).
Methods:  Methods: Primary astrocyte cultures were prepared from the cortex of postnatal rat pups. An astrocytes model of Huntington's disease was established using the astrocytes infected with adenovirus carrying coden gene of N-terminal 552 residues of Huntingtin. The protein levels of glutamate transporters GLT-1 and GLAST, the autophagic marker microtubule-associated protein 1A/1B-light chain 3 (LC3) and the autophagy substrate p62 in the astrocytes were examined using Western blotting. The mRNA expression levels of GLT-1 and GLAST in the astrocytes were determined using Real-time PCR. 3H]glutamate uptake by the astrocytes was measured with liquid scintillation counting.
Results:  The expression of mutant Htt-552 in the astrocytes significantly decreased both the mRNA and protein levels of GLT-1 but not those of GLAST. Furthermore, Htt-552 significantly reduced 3H]glutamate uptake by the astrocytes. Treatment with the autophagy inhibitor 3-MA (10 mmol/L) significantly increased the accumulation of mutant Htt-552, and reduced the expression of GLT-1 and 3H]glutamate uptake in the astrocytes. Treatment with the autophagy stimulator rapamycin (0.2 mg/mL) significantly reduced the accumulation of mutant Htt-552, and reversed the changes in GLT-1 expression and 3H]glutamate uptake in the astrocytes.
Conclusion:  Rapamcin, an autophagy stimulator, can prevent the suppression of GLT-1 expression and glutamate uptake by mutant Htt-552 in cultured astrocytes.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7039}
}