@article{APS7032,
author = {Duan-fang LIAO and Ning LU and Lin-sen LEI and Lin YU and Jian-xiong CHEN},
title = {Effects of gypenosides on mouse splenic lymphocyte transformation and DNA polymerase II activity in vitro},
journal = {Acta Pharmacologica Sinica},
volume = {16},
number = {4},
year = {2016},
keywords = {},
abstract = {AIM:
To study the effects of gypenosides (Gyp) on lymphocyte transformation and DNA polymerase II activity.
METHODS:
Lymphocyte transformation response was induced by concanavalin A and lipopolysaccharides respectively. The activity of DNA polymerase II and DNA synthesis were assayed with TTP and [3H]TdR incorporation respectively in mixed lymphocyte culture test.
RESULTS:
Gyp 2.5-20 mg L-1 enhanced splenic T- and B- cell transformation, increased the DNA synthesis and potentiated the activity of DNA polymerase II. However, Gyp > 40 mg L-1 showed contrary effects.
CONCLUSION:
Gyp regulated lymphocyte transformation and DNA synthesis by regulating DNA polymerase II activity.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7032}
}