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Platelet adhesion to cultured bovine cerebral microvascular endothelial cells by stimulation of platelet activating factor and antagonism of drugs

  
@article{APS7030,
	author = {Jin-hong HU and Du-xin SUN and Guo-qian ZENG and Ai-you LIN and Yao-cheng RUI},
	title = {Platelet adhesion to cultured bovine cerebral microvascular endothelial cells by stimulation of platelet activating factor and antagonism of drugs},
	journal = {Acta Pharmacologica Sinica},
	volume = {16},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {AIM:
To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular endothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB), 1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP), tetrandrine (Tet).
METHODS:
The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [3H]adenine labeling of rabbit blood platelet.
RESULTS:
The platelet adhesion to CMEC was increased by 36% vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0.1, 1, 10 mmol L-1 or DMPP 0.1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L-1 inhibited the PAF stimulating platelet adhesion to CMEC by 5.4%, 16.3%, 20.1%; 13.7%, 19.4%, 22.4%; and 5.5%, 23.1%, 32.6%, respectively.
CONCLUSION:
DMPP and Tet inhibited the PAF action in cerebral vascular system.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7030}
}