@article{APS6952,
author = {Li-Ya Qiao and Yu-Cang Du},
title = {Increase of mitogen-activated protein kinase activity in rat brain after injection of argipressin (4-8)},
journal = {Acta Pharmacologica Sinica},
volume = {18},
number = {4},
year = {2016},
keywords = {},
abstract = {AIM: To study the changes of mitogen-activated protein kinase (MAPK) activity in
rat brain stimulated by argipressin (4-8) (AVP (4-8)) (s.c.).
METHODS: Wistar rat was treated with AVP (4-8). MAPK activity in rat brain was
assayed by phosphorylation of its specific substrate myelin basic protein (MBP)
after the cytosolic extracts fractionated by MONO-Q anion-exchange
chromatography.
RESULTS: The activity of 44 kDa MAPK in rat brain was significantly enhanced by
AVP (4-8). The enhancement of MAPK activity in hippocampus was suppressed 80% by
ZDC(C)CPR, an antagonist of AVP(4-8). The level of 44 kDa MAPK protein had no
detectable differences between the administration groups and control. In rat
hippocampal slices, similar results were obtained.
CONCLUSION: The increasement of 44 kDa MAPK activity stimulated by AVP(4-8) was
mediated by its specific receptor, and was a short-period process activated by
protein phosphorylation, but not by protein expression. MAPK was involved in the
signal transduction pathway induced by AVP(4-8).},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6952}
}