@article{APS6946,
author = {Marta Sofía Valero and Diego Santos Fagundes and Laura Grasa and María Pilar Arruebo and Miguel Ángel Plaza and María Divina Murillo},
title = {Contractile effect of tachykinins on rabbit small intestine},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP).
Methods: Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath.
Results: The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122.
Conclusion: Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6946}
}