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Protective effects of tetrandrine on CCl4-injured hepatocytes.

  
@article{APS6875,
	author = {Xiao-Hong Chen and You-Mei Hu and Ya-Qin Liao},
	title = {Protective effects of tetrandrine on CCl4-injured hepatocytes.},
	journal = {Acta Pharmacologica Sinica},
	volume = {17},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {\"AIM: 

To study the protective effects of tetrandrine (Tet) on CCl4-injured hepatocytes.

METHODS: 

The cultured rat liver cells were poisoned by CCl4 (10 mmol.L-1). The membrane fluidity was detected by 1,6-diphenyl-1,3,5-hexatriene (DPH), a lipid probe. The Ca2+ concentration was assayed with Fura 2-AM, a sensitive calcium indicator.

RESULTS: 

Tet (1-1000 nmol.L-1) increased viability of liver cell (from 71% to 72%-89%), reduced lactate dehydrogenase (LDH) release, and malondialdehyde (MDA) formation. Tet prevented the heightening of the intracellular Ca2+ concentration and the attenuation of the membrane fluidity of liver cells (P < 0.05).

CONCLUSION: 

Tet had a protective effect on CCl4-injured hepatocytes by inhibiting the lipid peroxidation, improving the membrane fluidity, and lessening the Ca2+ concentration.
\"},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6875}
}