TY  - JOUR
AU  - Zhu Li
AU  - Wu Juan
AU  - Liao Hong
AU  - Gao Jing
AU  - Zhao Xiao-Ning
AU  - Zhang Zu-Xuan
PY  - 2016
TI  - Antagonistic effects of extract from leaves of ginkgo biloba on glutamate neurotoxicity
JF  - Acta Pharmacologica Sinica; Vol 18, No 4 (July 1997): Acta Pharmacologica Sinica
Y2  - 2016
KW  - 
N2  - AIM: To determine whether the extract of leaves of Ginkgo biloba L (EGb) and  several active constituents of EGb have protective effects against glutamate  (Glu)-induced neuronal damage.  METHODS: Microscopy and image analysis of nucleus areas in the arcuate nuclei  (AN) of mice were made. The neuronal viability in primary cultures from mouse  cerebral cortex was assessed using MTT [3-(4, 5-dimethylthiazol-2-yl)-2,  5-diphenyl tetrazolium bromide] staining and the intracellular free calcium  concentration ([Ca2+]i) of single neuron was measured using Fura-2.  RESULTS: EGb (2.5 mg.L-1) and its constituent ginkgolide B (Gin B, 2 mg.L-1)  protected the neuronal viability against Glu-induced injury, and prevented the  Glu-induced elevation in [Ca2+]i. EGb (3-10 mg.kg-1) attenuated the decrease of  nucleus areas in arcuate nuclei induced by Glu (1 g.kg-1, s.c.).  CONCLUSION: EGb and Gin B prevent neurons from Glu neurotoxicity through  reduction of the rise in [Ca2+]i.
UR  - http://www.chinaphar.com/article/view/6847