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Kinetic properties of nicotinic receptors in cultured rat sympathetic neurons from superior cervical ganglia

  
@article{APS6843,
	author = {Jian-Quan Zheng and Xiang-Ping He and Ai-Zhen Yang and Chuan-Gui Liu},
	title = {Kinetic properties of nicotinic receptors in cultured rat sympathetic neurons from superior cervical ganglia},
	journal = {Acta Pharmacologica Sinica},
	volume = {18},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {AIM: To analyze the kinetic properties of the effect of nicotine on nicotinic
acetylcholine receptors (nAChR) in the cultured sympathetic neurons from neonatal
rat superior cervical ganglia (SCG).
METHODS: The whole-cell recording method of patch-clamp technique was used to
record the currents induced by different concentrations of nicotine. The
concentration-response of nicotine was fitted with Clark equation.
RESULTS: Hill coefficient (1.097) was determined by fitting the nicotine
responses of neuronal nAChR with Clark equation. The theoretical values of
nicotine effect, calculated with Clark equation with H = 1, were basically
identical with the practically recorded currents.
CONCLUSIONS: Interaction of nicotine and nAChR in rat SCG fits a single binding
site model.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6843}
}