@article{APS6826,
author = {Feng-Hya Fu and Yu-Xia Wang and Feng-Zhen Li and Yan-Bin Xin and Man-Ji Sun},
title = {Monoclonal antibody 3F3 against conformational epitope of Torpedo acetylcholinesterase},
journal = {Acta Pharmacologica Sinica},
volume = {18},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM: To study the type of epitope of native Torpedo acetylcholinesterase (AChE)
directed by its monoclonal antibody (McAb) 3F3.
METHODS: Enzyme-linked immunosorbent assay (ELISA) was used for the assay of the
reaction between antigen and antibody.
RESULTS: McAb 3F3 immunoreacted well with the native AChE, but not with the
reduced- and alkylated-AChE (RA-AChE) at all. Soman did not interfere the binding
of 3F3 with AChE molecule. The synthesized 24-peptide containing the active
serine residue of the AChE active center did not react with McAb 3F3.
CONCLUSION: 3F3 is a monoclonal antibody against the conformational epitope of
Torpedo AChE active center, but dose not occupy the active serine residue of the
enzyme.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6826}
}