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Simvastatin combined with nifedipine enhances endothelial cell protection by inhibiting ROS generation and activating Akt phosphorylation

   
@article{APS6724,
	author = {Xiao-niao Chen and Jun Xu and Zhe Feng and Ming Fan and Jing-yao Han and Zhuo Yang},
	title = {Simvastatin combined with nifedipine enhances endothelial cell protection by inhibiting ROS generation and activating Akt phosphorylation},
	journal = {Acta Pharmacologica Sinica},
	volume = {31},
	number = {7},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the protective effects of simvastatin (Sim) combined with nifedipine (Nif) on endothelial cells and elucidate the action mechanism.
Methods: Human umbilical vein endothelial cells (HUVEC) were used. mRNA and protein levels were measured by using reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively. Intracellular calcium and reactive oxygen species (ROS) were detected using confocal microscopy. The Griess assay was used to evaluate nitric oxide (NO) release.
Results: Treatment of HUVEC with H2O2 100 μmol/L for 30 min inhibited the mRNA and protein expression of endothelial nitric oxide synthase (eNOS). With increased concentrations of Nif, eNOS mRNA and protein levels increased (P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6724}
}