@article{APS6697,
author = {Jian Li and Ying-zi He and Wen Li and Yun-zhen Shen and Yu-ru Li and Yun-feng Wang},
title = {A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery},
journal = {Acta Pharmacologica Sinica},
volume = {31},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity.
Methods: Poly (ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) was incorporated into polymer-lipid hybrid nanoparticles (PLN) to construct a PEG-DSPE modified long circulating PLN (L-PLN). The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry.
Results: The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes (N/P=10) was about 37.2%, which was higher than those transfected by PLN alone or commercial LipofectamineTM 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors.
Conclusion: L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6697}
}