@article{APS6647,
author = {Zi-jian Li and Ning Liu and Qi-de Han and You-yi Zhang},
title = {Proteome reference map and regulation network of neonatal rat cardiomyocyte},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {9},
year = {2016},
keywords = {},
abstract = {Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.
Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.
Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn.
Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6647}
}