@article{APS6605,
author = {Jue Tian and Zi Yan and Ye Wu and Su-li Zhang and Ke Wang and Xiu-rui Ma and Li Guo and Jin Wan Wang and Lin Zuo and Jing-yi Liu and Lin Quan and Hui-rong Liu},
title = {Inhibition of iNOS protects endothelial-dependent vasodilation in aged rats},
journal = {Acta Pharmacologica Sinica},
volume = {31},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: To examine whether iNOS contributes to endothelial dysfunction in aged rats.
Methods: Male Sprague Dawley rats were divided into three groups: young rats, aged rats treated with vehicle and aged rats treated with N-[3-(Aminomethyl) benzyl] acetamidine (1400W, 1 mg/kg, ip). Vasorelaxation was measured in isolated thoracic aorta. iNOS expression of thoracic aortic arteries was detected using immunohistochemistry and Western blot. Nitrotyrosine (a marker for peroxynitrite formation) content and expression in thoracic aortic tissue were determined using enzyme linked immunosorbent assay and immunohistochemistry.
Results: Maximal relaxation induced by acetylcholine (10-9 to 10-5 mol/L) in the aged rats treated with vehicle was significantly decreased (70%±15%, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6605}
}