@article{APS6603,
author = {Rui-xue Yang and Shan-ying Huang and Fang-fang Yan and Xiao-ting Lu and Yi-fan Xing and Yan Liu and Yun-fang Liu and Yu-xia Zhao},
title = {Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia},
journal = {Acta Pharmacologica Sinica},
volume = {31},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: To examine whether danshensu could protect vascular endothelia in a rat model of hyperhomocysteinemia.
Methods: The model was established by feeding rats with a methionine-rich diet (1 g·kg−1·d−1) for 3 months. Immediately following the discontinuation of methionine-rich diet, rats were treated with danshensu (67.5 mg·kg−1·d−1, po) or saline for 3 additional months. One group of rats receiving vitamin mixture (folic acid, vitamin B12 and vitamin B6) was included as a positive control. One group of rats not exposed to methionine-rich diet was also included as a blank control. The expression of tumor necrosis factor-alpha (TNF-alpha) and intercellular adhesion molecule-1 (ICAM-1) protein in the descending aorta was examined using immunohistochemistry and Western blot. Homocysteine and blood concentration of endothelin and nitric oxide (NO) was also examined.
Results: Methionine-rich diet resulted in accumulation of “foam cells”, up-regulated expression of TNF-alpha and ICAM-1 in the descending aorta, and significantly increased serum homocysteine. Plasma endothelin concentration was significantly increased; NO was decreased. Danshensu treatment, either simultaneous to methionine-rich diet or afterwards, attenuated the above mentioned changes.
Conclusion: Chronic treatment with danshensu could prevent/attenuate the formation of atherosclerosis. Potential mechanisms include inhibited expression of representative proinflammatory cytokines and adhesion molecules in arterial endothelia. Changes in homocysteine and circulating molecules that control vascular contraction/relaxation via endothelial cells (eg, endothelin and NO) were also implicated.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6603}
}