@article{APS6576,
author = {Bin Wu and Chong-Wen Wang and Jua-Rui Xu and Jub-Quan Zhu},
title = {Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CCl4},
journal = {Acta Pharmacologica Sinica},
volume = {18},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM: To study the effects of epidermal growth factor (EGF) on CCl4-induced
primary cultured hepatocytes injury.
METHODS: Alanine amino-transferase (AlaAT) and aspartate aminotransferase (AspAT)
activities and K+ concentractions were determined by the Auto-biochemistry Assay
System. Malondialdehyde (MDA) was determined by thiobarbituric acid method.
Radioactivity was determined by liquid scintillometry. Light microscopy and
electron microscopy were used.
RESULTS: EGF 40 micrograms.L-1 decreased CCl4 (10 mmol.L-1)-induced damages of
rat primary cultured hepatocytes by decreasing AlaAT and AspAT leakage and MDA
production, and promoted RNA and DNA synthesis, with a high positive correlation
between intracellular K+ leakage and DNA syntheses (r = 0.99, P < 0.01).
Cytopathological study showed that EGF decreased damage of liver cells.
CONCLUSION: EGF maintains the stability of cellular lipid membrane and promotes
syntheses of RNA and DNA of hepatocytes, and intracellular K+ transference is a
promotor of the message transmission of DNA synthesis.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6576}
}