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The impact of extracellular and intracellular Ca2+ on ethanol-induced smooth muscle contraction

   
@article{APS6479,
	author = {Naciye Yaktubay Döndaş and Mahir Kaplan and Derya Kaya and Ergin Şingirik},
	title = {The impact of extracellular and intracellular Ca2+ on ethanol-induced smooth muscle contraction},
	journal = {Acta Pharmacologica Sinica},
	volume = {30},
	number = {10},
	year = {2016},
	keywords = {},
	abstract = {Aim: To evaluate the impact of extracellular and intracellular Ca2+ on contractions induced by ethanol in smooth muscle.
Methods: Longitudinal smooth muscle strips were prepared from the gastric fundi of mice. The contractions of smooth muscle strips were recorded with an isometric force displacement transducer.
Results: Ethanol (164 mmol/L) produced reproducible contractions in isolated gastric fundal strips of mice. Although lidocaine (50 and 100 μmol/L), a local anesthetic agent, and hexamethonium (100 and 500 μmol/L), a ganglionic blocking agent, failed to affect these contractions, verapamil (1–50 μmol/L) and nifedipine (1–50 μmol/L), selective blockers of L-type Ca2+ channels, significantly inhibited the contractile responses of ethanol. Using a Ca2+-free medium nearly eliminated these contractions in the same tissue. Ryanodine (1–50 μmol/L) and ruthenium red (10–100 μmol/L), selective blockers of intracellular Ca2+ channels/ryanodine receptors; cyclopiazonic acid (CPA; 1–10 μmol/L), a selective inhibitor of sarcoplasmic reticulum (SR) Ca2+-ATPase; and caffeine (0.5–5 mmol/L), a depleting agent of intracellular Ca2+ stores, significantly inhibited the contractile responses induced by ethanol. In addition, the combination of caffeine (5 mmol/L) plus CPA (10 μmol/L), and ryanodine (10 μmol/L) plus CPA (10 μmol/L), caused further inhibition of contractions in response to ethanol. This inhibition was significantly different from those associated with caffeine, ryanodine or CPA. Furthermore the combination of caffeine (5 mmol/L), ryanodine (10 μmol/L) and CPA(10 μmol/L) eliminated the contractions induced by ethanol in isolated gastric fundal strips of mice.
Conclusion: Both extracellular and intracellular Ca2+ may have important roles in regulating contractions induced by ethanol in the mouse gastric fundus.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6479}
}