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Effects of alloxan on electric activity of mouse pancreatic B cells in vitro

  
@article{APS6450,
	author = {Xin-ming Shen and Qing-fen Su and Zi-wen Qian and Jing-ru Zhang},
	title = {Effects of alloxan on electric activity of mouse pancreatic B cells in vitro},
	journal = {Acta Pharmacologica Sinica},
	volume = {15},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {Microelectrode method for recording membrane potentials was used. It was observed that alloxan possessed stimulative and toxic dual effects on the electric activity of pancreatic B cells. A 10-min exposure to alloxan 14 mmol.L-1 in the perfusion medium without glucose caused a significant depolarization of B cells from -44 +/- 13 mV to -36 +/- 12 mV (n = 7, P < 0.05) and evoked spikes in B cells. But the spikes disappeared at 24 +/- 12 min (n = 8). In the presence of glucose 5.5 mmol.L-1 or 11.0 mmol.L-1, the dual effects became weaker or not obvious. After 10-min exposure to diazoxide (an ATP sensitive K+ channel opener) 0.8 mmol.L-1 in the perfusion medium with alloxan 14 mmol.L-1 but devoid of glucose, the amplitude of membrane potential went up from -31 +/- 11 mV to -48 +/- 21 mV and the mean value of spikes dropped from 31 +/- 24 spikes.min-1 to 5 +/- 5 spikes.min-1. It was suggested that the stimulative effect of alloxan was due to blocking of ATP sensitive K+ channel and a higher concentration of glucose could prevent B cells from alloxan injury.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6450}
}