@article{APS6410,
author = {Ling Lin and He-Yun Zhang and Hai-Ming Gu and Guo-Zhi Tang and Zu-Xuan Zhang and Jin-Xi Wang and Wei-Ning Zhang and Rong-San Chen},
title = {Nerve growth factors prevent glutamate toxicity in cortical neuronal cultures},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM: To determine if nerve growth factors (NGF) can protect against glutamate-induced cortical neuron damage.
METHODS: Neuron viability and lactate dehydrogenase (LDH) efflux in the bathing medium in primary cultures from 17-d-old mouse fetal cortex were measured to assay NGF effect. Imaging of the calcium indicator dye Fura-2 was used to measure the [Ca2+]i.
RESULTS: The LD50 for NGF-free glutamate was 0.2 mmol.L-1 (95% confidence limits 0-1.6 mmol.L-1). In the presence of NGF 60 micrograms.L-1, 59% of the neurons survived in glutamate 1.6 mmol.L-1. The protective effect afforded by NGF was maximal at 60 micrograms.L-1, at which it prevented the elevation in [Ca2+]i.
CONCLUSION: NGF protect cortical neurons against glutamate-induced toxicity via \"\"stabilizing\"\" [Ca2+]i level or suppression of the rise in [Ca2+]i.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6410}
}