@article{APS6329,
author = {Fei Chen and Tao Wang and Jia Wang and Zi-qiang Wang and Ming Qian},
title = {Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: The aim of the present study was to investigate the reversing effect of levistolide A (LA) on P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) in human breast carcinoma Bcap37/MDR1 cells.
Methods: After chemotherapeutic drugs (adriamycin or vincristine) used alone or in combination with LA, cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazo-lium bromide assay and cell cycle distribution by flow cytometry. RT-PCR was used to detect MDR1 gene transcription and the Western blot assay was used to assess P-gp expression and the cleavages of poly(ADP-ribose) polymerase and caspase-3. Apoptosis was detected by terminal transferase-mediated dUTP nick end-labeling assay. Moreover, the P-gp function was evaluated by the intracellular accumulation of the P-gp substrate detected by flow cytometry.
Results: We found the subcytotoxic doses of LA significantly enhanced adriamycin- or vincristine-induced G2/M arrest and apoptosis. These effects were consistent with the ability of LA to inhibit P-gp function. Moreover, LA dramatically enhanced the verapamil (VER) ability to reverse drug resistance.
Conclusion: LA has the potential to be developed as a novel P-gp modulator. Furthermore, the combination of LA and VER might represent a more sufficient but less toxic anti-MDR regimen.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6329}
}