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Aspergillus fumigatus conidia upregulates NOD2 protein expression both in vitro/ and in vivo

   
@article{APS6328,
	author = {Hui-jun Zhang and Jie-ming Qu and Chang-zhou Shao and Jing Zhang and Li-xian He and Zheng-hong Yuan},
	title = {Aspergillus fumigatus conidia upregulates NOD2 protein expression both in vitro/ and in vivo},
	journal = {Acta Pharmacologica Sinica},
	volume = {29},
	number = {10},
	year = {2016},
	keywords = {},
	abstract = {Aim: To determine if NOD2 is involved in host recognition of Aspergillus fumigatus (Af) conidia.
Methods: An Af conidia pulmonary infection murine model was established by intranasal inoculation of Af onidia suspensions. Protein levels of NOD2 in lung tissue were determined by immunohistochemistry. A549 and phorbol-12-myristate 13-acetate (PMA)-activated THP-1 cell lines were treated with heat-killed Af conidia, then the presence of NOD2 protein in these cell lines was detected by Western blotting. The ability of muramyl dipeptide (MDP) to induce the secretion of TNF-α after incubation with heat-killed Af conidia was measured by enzyme-linked immunosorbent assay.
Results: The expression of NOD2 protein in lung tissue increased after Af conidia infection. Heat-killed Af conidia significantly upregulated NOD2 protein expression in A549 cells and PMA-activated THP-1 cells. Additionally, Af conidia in conjuction with MDP, significantly increased the secretion of TNF-α in A549 cells and PMA-activated THP-1 cells.
Conclusion: Af conidia upregulates NOD2 protein expression in vitro/ and in vivo. These findings indicate that NOD2 protein may respond to Af conidia.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6328}
}