@article{APS6255,
author = {Zhi-Hong Liu and Lei-Shi Li and Wei-Xin Hu and Hong Zhou},
title = {Effect of emodin on c-myc proto-oncongen expression in cultured rat mesangial cells.},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {1},
year = {2016},
keywords = {},
abstract = {\"AIM:
To explore the possible molecular mechanism of emodin on the inhibition of glomerular mesangial cells (MC).
METHODS:
In rat MC culture, c-myc mRNA level was detected by dot-blotting as expressed by the dilutions of the total RNA on the visible dots.
RESULTS:
Low level c-myc mRNA was found in serum-free cultured quiescent MC. Addition of lipopolysaccharide (LPS 10 mg.L-1) induced a higher level expression of c-myc mRNA within 30 min, maximal expression at 2.5 h and persisted for 6 h. This over-expressing of c-myc mRNA was markedly suppressed by emodin (25 mg.L-1), the inhibition of emodin on c-myc mRNA expression was still seen at 6 h, and the greatest suppressive effect was at 2.5 h.
CONCLUSION:
Emodin participating in its down-regulatory effect of c-myc mRNA over-expression contributes to its inhibitory action on MC.
\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6255}
}